Journal: Neural Regeneration Research

Article Title: Extracellular matrix from human umbilical cord-derived mesenchymal stem cells as a scaffold for peripheral nerve regeneration

doi: 10.4103/1673-5374.187061

Figure Lengend Snippet: The mRNA and protein expression of related factors in control and ECM groups. (A) The mRNA levels (2 –ΔΔCt ) of S100, GFAP, P0, NGF, BDNF, GDNF, and VEGF in the control group (plated on non-coated dishes) and ECM group (plated on ECM-coated dishes). ** P < 0.01, *** P < 0.001, vs. control group. (B, C) Protein levels (gray value: target proteins/β-actin) of NGF, BDNF, GDNF, and VEGF in control and ECM groups. * P < 0.05, ** P < 0.01, vs . control group. Data are expressed as the mean ± SD. Statistical analysis was performed using two-sample t -test. ECM: Extracellular matrix; GFAP: glial fibrillary acidic protein; NGF: nerve growth factor; BDNF: brain-derived neurotrophic factor; GDNF: glial cell-derived neurotrophic factor; VEGF: vascular endothelial growth factor.

Article Snippet: Protein samples were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis, and then transferred onto polyvinylidene difluoride membranes and incubated with rabbit monoclonal antibody against NGF (1:500; Abcam), mouse monoclonal antibody against BDNF (1:200; Abcam), rabbit polyclonal antibody against GDNF (1:200; Abcam), and mouse monoclonal antibody against VEGF (1:200; Abcam) for 3 hours at 37°C.

Techniques: Expressing, Control, Derivative Assay

Journal: Molecular Medicine Reports

Article Title: Neurotrophin expression and laryngeal muscle pathophysiology following recurrent laryngeal nerve transection

doi: 10.3892/mmr.2015.4684

Figure Lengend Snippet: Expression of BDNF and GDNF in TA and PCA muscles. (A) BDNF expression levels were reduced over time in the right TA, and were significantly reduced at 3 weeks following injury in the right PCA. (B) GDNF levels were similar between the TA and PCA muscles from 3–10 weeks. At 16 weeks, the expression levels were greater in the right PCA muscle compared with the right TA muscle. * P<0.05 vs. the control group. BDNF, brain-derived neurotrophic factor; GDNF, glial cell line-derived neurotrophic factor; PCA, posterior cricoarytenoid; TA, thyroarytenoid.

Article Snippet: The sections were then incubated with a rabbit monoclonal antibody against BDNF (dilution, 1:75; ab108319; Abcam, Cambridge, MA, USA) or a rabbit polyclonal antibody against GDNF (dilution, 1:100; sc-328; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), overnight at 4°C, followed by incubation for 15 min at 37°C with the horseradish peroxidase-conjugated secondary antibody (GK500711; Genetic Technology Co., Ltd., Shanghai, China) according to the manufacturer's instructions.

Techniques: Expressing, Muscles, Control, Derivative Assay